NASH caused hepatomegaly, fibrosis, and inflammation in all mice, with aged mice showing increased heart weight, left ventricular volumes, and subtle systolic and diastolic dysfunction, thus NASH tends to worsen age-related cardiac dysfunction [37]
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( B O ) Flow cytometry analysis of CD45 + cells ( B ), neutrophils ( C ), Ly6G hi monocytes ( D ), total macrophages ( E ), CD206 + CD301b + M2 macrophages ( F ), Arg + M2 macrophages ( G ), iNOS + M1 macrophages ( H ), CD45 + CD4 + T cells ( I ), Gata3 Th2 cells ( J ), Rort Th17 cells ( K ), T-bet Th1 cells ( L ), Foxp3 + Tregs ( M ), IL-17 + Tregs ( N ), and Rort + Foxp3 + Tregs ( O ) in eWAT of 18- to 22-week-old male WT and Bbs5 / mice ( n = 46 per group)
